Bibliothèque Électronique Lac Saint-Pierre

Detection and confirmation of saxitoxin analogues in freshwater benthic Lyngbya wollei algae collected in the St. Lawrence River (Canada) by liquid chromatography–tandem mass spectrometry

Lajeunesse, A. et Segura, P.A. et Gélinas, M. et Hudon, C. et Thomas, K. et Quilliam, M.A. et Gagnon, C. (2012). Detection and confirmation of saxitoxin analogues in freshwater benthic Lyngbya wollei algae collected in the St. Lawrence River (Canada) by liquid chromatography–tandem mass spectrometry. Journal of Chromatography A , 1219 . p. 93-103. DOI: 10.1016/j.chroma.2011.10.092.

Ce document n'est pas hébergé sur Bibliothèque Électronique Lac Saint-Pierre.

Résumé

The presence of cyanotoxins in benthic Lyngbya wollei algae samples collected in a fluvial lake along the St. Lawrence River, Canada, was investigated using a multi-toxins method. Hydrophilic interaction liquid chromatography (HILIC) and reverse phased liquid chromatography (RPLC) were coupled to triple quadrupole mass spectrometry (LC-QqQMS) for quantification and to quadrupole-time of flight mass spectrometry (LC-QqTOFMS) for screening and confirmation. The presence of two saxitoxin analogues, LWTX-1 and LWTX-6, was confirmed in benthic Lyngbya wollei algae samples. Concentration of LWTX-1 was between 209±5 and 279±9µgg−1. No other targeted cyanotoxin (such as anatoxin-a, nodularin, microcystin-LR, microcystins-RR and saxitoxin) was found in the samples. The presence of LWTX-6 was observed by using a screening approach based on an in-house database of cyanotoxins, an algorithm of identification and high resolution mass spectrometry measurements on the precursor and product ions. This work demonstrates the need for more research on the fate of benthic cyanotoxins in aquatic ecosystems such the St. Lawrence River.

Type de document: Article scientifique
Statut du texte intégral: Autre
Mots-clés libres: Benthic algae, Cyanotoxins, PSP toxins, Lyngbya wollei, LC–MS/MS, HILIC
Sujets: 2. Milieu physique > 2.4. Hydrologie
3. Végétation, milieux humides
Date de dépôt: 11 août 2016 18:31
Dernière modification: 11 août 2016 18:31
URI: http://belsp.uqtr.ca/id/eprint/320

Actions (Identification requise)

Dernière vérification avant le dépôt Dernière vérification avant le dépôt
Coopérative de solidarité de la Réserve de la biosphère du Lac-Saint-Pierre
Concepteur de la BELSP : André Barabé, Ph. D.